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KMID : 0903619980390010119
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Journal of the Korean Society for Horticultural Science
1998 Volume.39 No. 1 p.119 ~ p.124
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Cell Fusion of Bacillus subtilis and Corynebacterium glutamicum , Characteristics of Fusants and Development of Effective Rhizobacteria for Hydroponics
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Abstract
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To develop strains of effective rhizobacteria for hydroponics, intergeneric cell fusions between Bacillus subtilis having a strong antifungal activities against the root-infected pathogens and Corynebacterium glutamicum producing glutamic acid were done. In case of C. glutamicum K, cells were cultured in the minimal medium containing 1.5% glycine. The cells grown at the mid-exponential growth phase were treated with 0.3 unit/mL of penicillin G and 350 g/mL lysozyme, resulting in 91.7% protoplast formation. Fourteen % of cell wall regeneration was obtained in regeneration medium supplemented with 3% polyvinyl pyrrolidone. For B. subtilis R, 1.0% glycine and 200 g/mL lysozyme showed 99.9% protoplast formation. In intergeneric protoplast fusion between B. subtilis and C. glutamicum, 7.91×10^(-7) of fusion frequency per regenerated cell was obtained using 30% (w/v) PEG (M.W. 6,000) in fusion solution (pH 10.5). The fusants were almost the same as those of Bacillus subtilis in antagonistic activities against root-infected pathogens.
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